Energy metabolism in human erythrocytes

Energy metabolism in human erythrocytes. II. Effects of glucose depletion.

Normal red cells were incubated in the absence of glucose to develop a system in which total adenosine triphosphate (ATP) turnover could be assessed. After 1 hr, the triose pool had been completely consumed. Thereafter, the metabolism of 2,3-diphosphoglycerate (DPG) to pyruvate and lactate was the sole significant source of ATP synthesis.10(-3)M CuCl(2), which did not enter the cells, diminishe...

Energy metabolism in human erythrocytes. I. Effects of sodium fluoride.

Exposure of red cells to fluoride produces a variety of metabolic alterations, most of which are based upon the secondary effects of enolase inhibition, which reduces pyruvate synthesis and interferes with the regeneration of diphosphopyridine nucleotide (NAD). Adenosine triphosphate (ATP) is consumed in the hexokinase and phosphofructokinase reactions but is not regenerated since the deficienc...

Adenosine Metabolism in Human Erythrocytes

Energy metabolism in human erythrocytes: the role of phosphoglycerate kinase in cation transport.

Three models of disturbed erythrocyte metabolism, triose-depleted normal, phosphoglycerate kinase (PGK)-deficient, and pyruvate kinase (PK)-deficient cells, have been studied to examine further the role of PGK in erythrocyte cation transport. Sodium (Na-+) and potassium (K-+) transport were reduced only in cells fully depleted of triose. In such cells the PGK step presumably was inoperative due...

Magnesium metabolism of human and rabbit erythrocytes.

By STANLEY GINSBURG, JAMES G. Sxirni, FREEMAN M. GINSBURG, JACQUELINE Z. REARDON AND JERIIY K. AIKAWA T HE CURRENT PAUCITY of information concerning magnesium metabolism in erythrocytes is due in part to the lack of a reliable method for determining magnesium in red cells, and in part to the fact that a radioactive isotope of magnesium suitable for tracer studies has only recently become availa...